pparγ protein Search Results


recombinant pparγ  (Sino Biological)
93
Sino Biological recombinant pparγ
Caspase‐6 cleaves <t>PPARγ</t> and SP1 to control ATGL expression in adipocytes. (A, B) Immunoblot of PPARγ and ATGL proteins in eWAT (A) and iWAT (B) of WT and C6KO mice fed 60% HFD for 12 weeks (n = 4). Two tailed unpaired Student's t ‐test. (C) In vitro cleavage of <t>recombinant</t> PPARγ by active caspase‐6. Immunoblot of PPARγ. (D) Immunoblot of lysates from 3T3‐L1 adipocytes treated with cycloheximide (5 µg/ml) and TNFα (25 ng/ml) for indicated time with or without pretreatment of Emricasan (50 µg/ml) for 1 hr. (E, F) Immunoblot SP1 protein in eWAT (E) and iWAT (F) of WT and C6KO mice fed HFD for 12 weeks (n = 3). Two tailed unpaired Student's t ‐test. (G) In vitro cleavage of recombinant SP1 by active caspase‐6. Coomassie blue staining of gel. (H) Immunoblot of lysates from 3T3‐L1 adipocytes treated with cycloheximide (CHX, 5 µg/ml) and TNFα (25 ng/ml) for indicated time with or without pretreatment of Emricasan (50 µg/ml) for 1 hr. (I) Immunoblot of lysates from ex vivo cultured eWAT from WT or C6KO, treated with CHX (15 µg/ml) and TNFα (75 ng/ml) for indicated time. (J) Pnpla2 expression in 3T3‐L1 adipocytes treated with Rosiglitazone (5µM) or T0070907 (100 nM) in the presence or absence of Mithramycin (10 µM) for 6 h (n = 3). Two tailed unpaired Student's t ‐test. (K) In vitro cleavage of WT and mutant (D69E) PPARγ2 expressed in HEK293T by recombinant active caspase‐6. (L) In vitro cleavage of WT and mutant (D185E) SP1 expressed in HEK293T by recombinant active caspase‐6. (M) PPARγ activity reporter assay in HEK293T cell expressing PPRE‐H2B‐eGFP along with WT PPARγ or PPARγ D69E mutant in the absence or presence of CHX‐TNFα treatment (n = 8). Two tailed unpaired Student's t ‐test. (N‐V) Pearson correlation between CASP6 and PPARγ target genes in human adipose tissues ( GSE245948 ): Correlation matrix (N), AQP7 (O), CPT1B (P), FADS2 (Q), ME3 (R), PLIN4 (S), PCK2 (T), SLC27A1 (U), and SLC27A4 (V). (n = 76). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Recombinant Pparγ, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
recombinant pparγ - by Bioz Stars, 2026-06
93/100 stars
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recombinant pparγ protein  (Active Motif)
90
Active Motif recombinant pparγ protein
Caspase‐6 cleaves <t>PPARγ</t> and SP1 to control ATGL expression in adipocytes. (A, B) Immunoblot of PPARγ and ATGL proteins in eWAT (A) and iWAT (B) of WT and C6KO mice fed 60% HFD for 12 weeks (n = 4). Two tailed unpaired Student's t ‐test. (C) In vitro cleavage of <t>recombinant</t> PPARγ by active caspase‐6. Immunoblot of PPARγ. (D) Immunoblot of lysates from 3T3‐L1 adipocytes treated with cycloheximide (5 µg/ml) and TNFα (25 ng/ml) for indicated time with or without pretreatment of Emricasan (50 µg/ml) for 1 hr. (E, F) Immunoblot SP1 protein in eWAT (E) and iWAT (F) of WT and C6KO mice fed HFD for 12 weeks (n = 3). Two tailed unpaired Student's t ‐test. (G) In vitro cleavage of recombinant SP1 by active caspase‐6. Coomassie blue staining of gel. (H) Immunoblot of lysates from 3T3‐L1 adipocytes treated with cycloheximide (CHX, 5 µg/ml) and TNFα (25 ng/ml) for indicated time with or without pretreatment of Emricasan (50 µg/ml) for 1 hr. (I) Immunoblot of lysates from ex vivo cultured eWAT from WT or C6KO, treated with CHX (15 µg/ml) and TNFα (75 ng/ml) for indicated time. (J) Pnpla2 expression in 3T3‐L1 adipocytes treated with Rosiglitazone (5µM) or T0070907 (100 nM) in the presence or absence of Mithramycin (10 µM) for 6 h (n = 3). Two tailed unpaired Student's t ‐test. (K) In vitro cleavage of WT and mutant (D69E) PPARγ2 expressed in HEK293T by recombinant active caspase‐6. (L) In vitro cleavage of WT and mutant (D185E) SP1 expressed in HEK293T by recombinant active caspase‐6. (M) PPARγ activity reporter assay in HEK293T cell expressing PPRE‐H2B‐eGFP along with WT PPARγ or PPARγ D69E mutant in the absence or presence of CHX‐TNFα treatment (n = 8). Two tailed unpaired Student's t ‐test. (N‐V) Pearson correlation between CASP6 and PPARγ target genes in human adipose tissues ( GSE245948 ): Correlation matrix (N), AQP7 (O), CPT1B (P), FADS2 (Q), ME3 (R), PLIN4 (S), PCK2 (T), SLC27A1 (U), and SLC27A4 (V). (n = 76). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Recombinant Pparγ Protein, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant pparγ protein/product/Active Motif
Average 90 stars, based on 1 article reviews
recombinant pparγ protein - by Bioz Stars, 2026-06
90/100 stars
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pathhunter® pparγ protein interaction assay  (DiscoverX corporation)
90
DiscoverX corporation pathhunter® pparγ protein interaction assay
Caspase‐6 cleaves <t>PPARγ</t> and SP1 to control ATGL expression in adipocytes. (A, B) Immunoblot of PPARγ and ATGL proteins in eWAT (A) and iWAT (B) of WT and C6KO mice fed 60% HFD for 12 weeks (n = 4). Two tailed unpaired Student's t ‐test. (C) In vitro cleavage of <t>recombinant</t> PPARγ by active caspase‐6. Immunoblot of PPARγ. (D) Immunoblot of lysates from 3T3‐L1 adipocytes treated with cycloheximide (5 µg/ml) and TNFα (25 ng/ml) for indicated time with or without pretreatment of Emricasan (50 µg/ml) for 1 hr. (E, F) Immunoblot SP1 protein in eWAT (E) and iWAT (F) of WT and C6KO mice fed HFD for 12 weeks (n = 3). Two tailed unpaired Student's t ‐test. (G) In vitro cleavage of recombinant SP1 by active caspase‐6. Coomassie blue staining of gel. (H) Immunoblot of lysates from 3T3‐L1 adipocytes treated with cycloheximide (CHX, 5 µg/ml) and TNFα (25 ng/ml) for indicated time with or without pretreatment of Emricasan (50 µg/ml) for 1 hr. (I) Immunoblot of lysates from ex vivo cultured eWAT from WT or C6KO, treated with CHX (15 µg/ml) and TNFα (75 ng/ml) for indicated time. (J) Pnpla2 expression in 3T3‐L1 adipocytes treated with Rosiglitazone (5µM) or T0070907 (100 nM) in the presence or absence of Mithramycin (10 µM) for 6 h (n = 3). Two tailed unpaired Student's t ‐test. (K) In vitro cleavage of WT and mutant (D69E) PPARγ2 expressed in HEK293T by recombinant active caspase‐6. (L) In vitro cleavage of WT and mutant (D185E) SP1 expressed in HEK293T by recombinant active caspase‐6. (M) PPARγ activity reporter assay in HEK293T cell expressing PPRE‐H2B‐eGFP along with WT PPARγ or PPARγ D69E mutant in the absence or presence of CHX‐TNFα treatment (n = 8). Two tailed unpaired Student's t ‐test. (N‐V) Pearson correlation between CASP6 and PPARγ target genes in human adipose tissues ( GSE245948 ): Correlation matrix (N), AQP7 (O), CPT1B (P), FADS2 (Q), ME3 (R), PLIN4 (S), PCK2 (T), SLC27A1 (U), and SLC27A4 (V). (n = 76). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Pathhunter® Pparγ Protein Interaction Assay, supplied by DiscoverX corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pathhunter® pparγ protein interaction assay/product/DiscoverX corporation
Average 90 stars, based on 1 article reviews
pathhunter® pparγ protein interaction assay - by Bioz Stars, 2026-06
90/100 stars
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pparγ protein  (Lonza)
90
Lonza pparγ protein
Caspase‐6 cleaves <t>PPARγ</t> and SP1 to control ATGL expression in adipocytes. (A, B) Immunoblot of PPARγ and ATGL proteins in eWAT (A) and iWAT (B) of WT and C6KO mice fed 60% HFD for 12 weeks (n = 4). Two tailed unpaired Student's t ‐test. (C) In vitro cleavage of <t>recombinant</t> PPARγ by active caspase‐6. Immunoblot of PPARγ. (D) Immunoblot of lysates from 3T3‐L1 adipocytes treated with cycloheximide (5 µg/ml) and TNFα (25 ng/ml) for indicated time with or without pretreatment of Emricasan (50 µg/ml) for 1 hr. (E, F) Immunoblot SP1 protein in eWAT (E) and iWAT (F) of WT and C6KO mice fed HFD for 12 weeks (n = 3). Two tailed unpaired Student's t ‐test. (G) In vitro cleavage of recombinant SP1 by active caspase‐6. Coomassie blue staining of gel. (H) Immunoblot of lysates from 3T3‐L1 adipocytes treated with cycloheximide (CHX, 5 µg/ml) and TNFα (25 ng/ml) for indicated time with or without pretreatment of Emricasan (50 µg/ml) for 1 hr. (I) Immunoblot of lysates from ex vivo cultured eWAT from WT or C6KO, treated with CHX (15 µg/ml) and TNFα (75 ng/ml) for indicated time. (J) Pnpla2 expression in 3T3‐L1 adipocytes treated with Rosiglitazone (5µM) or T0070907 (100 nM) in the presence or absence of Mithramycin (10 µM) for 6 h (n = 3). Two tailed unpaired Student's t ‐test. (K) In vitro cleavage of WT and mutant (D69E) PPARγ2 expressed in HEK293T by recombinant active caspase‐6. (L) In vitro cleavage of WT and mutant (D185E) SP1 expressed in HEK293T by recombinant active caspase‐6. (M) PPARγ activity reporter assay in HEK293T cell expressing PPRE‐H2B‐eGFP along with WT PPARγ or PPARγ D69E mutant in the absence or presence of CHX‐TNFα treatment (n = 8). Two tailed unpaired Student's t ‐test. (N‐V) Pearson correlation between CASP6 and PPARγ target genes in human adipose tissues ( GSE245948 ): Correlation matrix (N), AQP7 (O), CPT1B (P), FADS2 (Q), ME3 (R), PLIN4 (S), PCK2 (T), SLC27A1 (U), and SLC27A4 (V). (n = 76). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Pparγ Protein, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pparγ protein/product/Lonza
Average 90 stars, based on 1 article reviews
pparγ protein - by Bioz Stars, 2026-06
90/100 stars
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gst-pparγ protein  (Boston Biochem)
90
Boston Biochem gst-pparγ protein
Caspase‐6 cleaves <t>PPARγ</t> and SP1 to control ATGL expression in adipocytes. (A, B) Immunoblot of PPARγ and ATGL proteins in eWAT (A) and iWAT (B) of WT and C6KO mice fed 60% HFD for 12 weeks (n = 4). Two tailed unpaired Student's t ‐test. (C) In vitro cleavage of <t>recombinant</t> PPARγ by active caspase‐6. Immunoblot of PPARγ. (D) Immunoblot of lysates from 3T3‐L1 adipocytes treated with cycloheximide (5 µg/ml) and TNFα (25 ng/ml) for indicated time with or without pretreatment of Emricasan (50 µg/ml) for 1 hr. (E, F) Immunoblot SP1 protein in eWAT (E) and iWAT (F) of WT and C6KO mice fed HFD for 12 weeks (n = 3). Two tailed unpaired Student's t ‐test. (G) In vitro cleavage of recombinant SP1 by active caspase‐6. Coomassie blue staining of gel. (H) Immunoblot of lysates from 3T3‐L1 adipocytes treated with cycloheximide (CHX, 5 µg/ml) and TNFα (25 ng/ml) for indicated time with or without pretreatment of Emricasan (50 µg/ml) for 1 hr. (I) Immunoblot of lysates from ex vivo cultured eWAT from WT or C6KO, treated with CHX (15 µg/ml) and TNFα (75 ng/ml) for indicated time. (J) Pnpla2 expression in 3T3‐L1 adipocytes treated with Rosiglitazone (5µM) or T0070907 (100 nM) in the presence or absence of Mithramycin (10 µM) for 6 h (n = 3). Two tailed unpaired Student's t ‐test. (K) In vitro cleavage of WT and mutant (D69E) PPARγ2 expressed in HEK293T by recombinant active caspase‐6. (L) In vitro cleavage of WT and mutant (D185E) SP1 expressed in HEK293T by recombinant active caspase‐6. (M) PPARγ activity reporter assay in HEK293T cell expressing PPRE‐H2B‐eGFP along with WT PPARγ or PPARγ D69E mutant in the absence or presence of CHX‐TNFα treatment (n = 8). Two tailed unpaired Student's t ‐test. (N‐V) Pearson correlation between CASP6 and PPARγ target genes in human adipose tissues ( GSE245948 ): Correlation matrix (N), AQP7 (O), CPT1B (P), FADS2 (Q), ME3 (R), PLIN4 (S), PCK2 (T), SLC27A1 (U), and SLC27A4 (V). (n = 76). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Gst Pparγ Protein, supplied by Boston Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gst-pparγ protein/product/Boston Biochem
Average 90 stars, based on 1 article reviews
gst-pparγ protein - by Bioz Stars, 2026-06
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primer for proliferator-activated receptor-alpha (pparα), pparγ, sterol regulatory element-binding protein-1 (srebp1) and tumor necrosis factor-alpha (tnfα)  (Metabion International AG)
90
Metabion International AG primer for proliferator-activated receptor-alpha (pparα), pparγ, sterol regulatory element-binding protein-1 (srebp1) and tumor necrosis factor-alpha (tnfα)
Caspase‐6 cleaves <t>PPARγ</t> and SP1 to control ATGL expression in adipocytes. (A, B) Immunoblot of PPARγ and ATGL proteins in eWAT (A) and iWAT (B) of WT and C6KO mice fed 60% HFD for 12 weeks (n = 4). Two tailed unpaired Student's t ‐test. (C) In vitro cleavage of <t>recombinant</t> PPARγ by active caspase‐6. Immunoblot of PPARγ. (D) Immunoblot of lysates from 3T3‐L1 adipocytes treated with cycloheximide (5 µg/ml) and TNFα (25 ng/ml) for indicated time with or without pretreatment of Emricasan (50 µg/ml) for 1 hr. (E, F) Immunoblot SP1 protein in eWAT (E) and iWAT (F) of WT and C6KO mice fed HFD for 12 weeks (n = 3). Two tailed unpaired Student's t ‐test. (G) In vitro cleavage of recombinant SP1 by active caspase‐6. Coomassie blue staining of gel. (H) Immunoblot of lysates from 3T3‐L1 adipocytes treated with cycloheximide (CHX, 5 µg/ml) and TNFα (25 ng/ml) for indicated time with or without pretreatment of Emricasan (50 µg/ml) for 1 hr. (I) Immunoblot of lysates from ex vivo cultured eWAT from WT or C6KO, treated with CHX (15 µg/ml) and TNFα (75 ng/ml) for indicated time. (J) Pnpla2 expression in 3T3‐L1 adipocytes treated with Rosiglitazone (5µM) or T0070907 (100 nM) in the presence or absence of Mithramycin (10 µM) for 6 h (n = 3). Two tailed unpaired Student's t ‐test. (K) In vitro cleavage of WT and mutant (D69E) PPARγ2 expressed in HEK293T by recombinant active caspase‐6. (L) In vitro cleavage of WT and mutant (D185E) SP1 expressed in HEK293T by recombinant active caspase‐6. (M) PPARγ activity reporter assay in HEK293T cell expressing PPRE‐H2B‐eGFP along with WT PPARγ or PPARγ D69E mutant in the absence or presence of CHX‐TNFα treatment (n = 8). Two tailed unpaired Student's t ‐test. (N‐V) Pearson correlation between CASP6 and PPARγ target genes in human adipose tissues ( GSE245948 ): Correlation matrix (N), AQP7 (O), CPT1B (P), FADS2 (Q), ME3 (R), PLIN4 (S), PCK2 (T), SLC27A1 (U), and SLC27A4 (V). (n = 76). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Primer For Proliferator Activated Receptor Alpha (Pparα), Pparγ, Sterol Regulatory Element Binding Protein 1 (Srebp1) And Tumor Necrosis Factor Alpha (Tnfα), supplied by Metabion International AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primer for proliferator-activated receptor-alpha (pparα), pparγ, sterol regulatory element-binding protein-1 (srebp1) and tumor necrosis factor-alpha (tnfα)/product/Metabion International AG
Average 90 stars, based on 1 article reviews
primer for proliferator-activated receptor-alpha (pparα), pparγ, sterol regulatory element-binding protein-1 (srebp1) and tumor necrosis factor-alpha (tnfα) - by Bioz Stars, 2026-06
90/100 stars
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ppargamma protein  (Jennewein Biotechnologie GmbH)
90
Jennewein Biotechnologie GmbH ppargamma protein
Caspase‐6 cleaves <t>PPARγ</t> and SP1 to control ATGL expression in adipocytes. (A, B) Immunoblot of PPARγ and ATGL proteins in eWAT (A) and iWAT (B) of WT and C6KO mice fed 60% HFD for 12 weeks (n = 4). Two tailed unpaired Student's t ‐test. (C) In vitro cleavage of <t>recombinant</t> PPARγ by active caspase‐6. Immunoblot of PPARγ. (D) Immunoblot of lysates from 3T3‐L1 adipocytes treated with cycloheximide (5 µg/ml) and TNFα (25 ng/ml) for indicated time with or without pretreatment of Emricasan (50 µg/ml) for 1 hr. (E, F) Immunoblot SP1 protein in eWAT (E) and iWAT (F) of WT and C6KO mice fed HFD for 12 weeks (n = 3). Two tailed unpaired Student's t ‐test. (G) In vitro cleavage of recombinant SP1 by active caspase‐6. Coomassie blue staining of gel. (H) Immunoblot of lysates from 3T3‐L1 adipocytes treated with cycloheximide (CHX, 5 µg/ml) and TNFα (25 ng/ml) for indicated time with or without pretreatment of Emricasan (50 µg/ml) for 1 hr. (I) Immunoblot of lysates from ex vivo cultured eWAT from WT or C6KO, treated with CHX (15 µg/ml) and TNFα (75 ng/ml) for indicated time. (J) Pnpla2 expression in 3T3‐L1 adipocytes treated with Rosiglitazone (5µM) or T0070907 (100 nM) in the presence or absence of Mithramycin (10 µM) for 6 h (n = 3). Two tailed unpaired Student's t ‐test. (K) In vitro cleavage of WT and mutant (D69E) PPARγ2 expressed in HEK293T by recombinant active caspase‐6. (L) In vitro cleavage of WT and mutant (D185E) SP1 expressed in HEK293T by recombinant active caspase‐6. (M) PPARγ activity reporter assay in HEK293T cell expressing PPRE‐H2B‐eGFP along with WT PPARγ or PPARγ D69E mutant in the absence or presence of CHX‐TNFα treatment (n = 8). Two tailed unpaired Student's t ‐test. (N‐V) Pearson correlation between CASP6 and PPARγ target genes in human adipose tissues ( GSE245948 ): Correlation matrix (N), AQP7 (O), CPT1B (P), FADS2 (Q), ME3 (R), PLIN4 (S), PCK2 (T), SLC27A1 (U), and SLC27A4 (V). (n = 76). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Ppargamma Protein, supplied by Jennewein Biotechnologie GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ppargamma protein/product/Jennewein Biotechnologie GmbH
Average 90 stars, based on 1 article reviews
ppargamma protein - by Bioz Stars, 2026-06
90/100 stars
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protein human ppar γ (hppar γ)  (Flarebio Biotech)
90
Flarebio Biotech protein human ppar γ (hppar γ)
Structures of DBDF2,6T and hPPAR γ protein: (a) 2D structure of DBDF2,6T and (b) 3D structure of <t>PPAR</t> <t>γ</t> (4a4w.pdb).
Protein Human Ppar γ (Hppar γ), supplied by Flarebio Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protein human ppar γ (hppar γ)/product/Flarebio Biotech
Average 90 stars, based on 1 article reviews
protein human ppar γ (hppar γ) - by Bioz Stars, 2026-06
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ppar gamma pparg nm 005037 human recombinant protein  (OriGene)
N/A
Recombinant protein of human peroxisome proliferator activated receptor gamma PPARG transcript variant 4
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ppar gamma protein (oprb00116)  (Aviva Systems)
N/A
This nuclear receptor functions as a transcription factor and plays an important role in controlling differentiation and fatty acid metabolism. Activation of this receptor appears to be vital in modulating the expression of metabolic control
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ppar gamma/nr1c3 recombinant protein antigen  (Novus Biologicals)
N/A
PPAR gamma/NR1C3 Recombinant Protein Antigen
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ppar gamma protein (oprb00117)  (Aviva Systems)
N/A
This nuclear receptor functions as a transcription factor and plays an important role in controlling differentiation and fatty acid metabolism. Activation of this receptor appears to be vital in modulating the expression of metabolic control
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Image Search Results


Caspase‐6 cleaves PPARγ and SP1 to control ATGL expression in adipocytes. (A, B) Immunoblot of PPARγ and ATGL proteins in eWAT (A) and iWAT (B) of WT and C6KO mice fed 60% HFD for 12 weeks (n = 4). Two tailed unpaired Student's t ‐test. (C) In vitro cleavage of recombinant PPARγ by active caspase‐6. Immunoblot of PPARγ. (D) Immunoblot of lysates from 3T3‐L1 adipocytes treated with cycloheximide (5 µg/ml) and TNFα (25 ng/ml) for indicated time with or without pretreatment of Emricasan (50 µg/ml) for 1 hr. (E, F) Immunoblot SP1 protein in eWAT (E) and iWAT (F) of WT and C6KO mice fed HFD for 12 weeks (n = 3). Two tailed unpaired Student's t ‐test. (G) In vitro cleavage of recombinant SP1 by active caspase‐6. Coomassie blue staining of gel. (H) Immunoblot of lysates from 3T3‐L1 adipocytes treated with cycloheximide (CHX, 5 µg/ml) and TNFα (25 ng/ml) for indicated time with or without pretreatment of Emricasan (50 µg/ml) for 1 hr. (I) Immunoblot of lysates from ex vivo cultured eWAT from WT or C6KO, treated with CHX (15 µg/ml) and TNFα (75 ng/ml) for indicated time. (J) Pnpla2 expression in 3T3‐L1 adipocytes treated with Rosiglitazone (5µM) or T0070907 (100 nM) in the presence or absence of Mithramycin (10 µM) for 6 h (n = 3). Two tailed unpaired Student's t ‐test. (K) In vitro cleavage of WT and mutant (D69E) PPARγ2 expressed in HEK293T by recombinant active caspase‐6. (L) In vitro cleavage of WT and mutant (D185E) SP1 expressed in HEK293T by recombinant active caspase‐6. (M) PPARγ activity reporter assay in HEK293T cell expressing PPRE‐H2B‐eGFP along with WT PPARγ or PPARγ D69E mutant in the absence or presence of CHX‐TNFα treatment (n = 8). Two tailed unpaired Student's t ‐test. (N‐V) Pearson correlation between CASP6 and PPARγ target genes in human adipose tissues ( GSE245948 ): Correlation matrix (N), AQP7 (O), CPT1B (P), FADS2 (Q), ME3 (R), PLIN4 (S), PCK2 (T), SLC27A1 (U), and SLC27A4 (V). (n = 76). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Advanced Science

Article Title: Caspase‐6 Controls Lipid and Energy Metabolism in Diet‐Induced Obesity

doi: 10.1002/advs.202514784

Figure Lengend Snippet: Caspase‐6 cleaves PPARγ and SP1 to control ATGL expression in adipocytes. (A, B) Immunoblot of PPARγ and ATGL proteins in eWAT (A) and iWAT (B) of WT and C6KO mice fed 60% HFD for 12 weeks (n = 4). Two tailed unpaired Student's t ‐test. (C) In vitro cleavage of recombinant PPARγ by active caspase‐6. Immunoblot of PPARγ. (D) Immunoblot of lysates from 3T3‐L1 adipocytes treated with cycloheximide (5 µg/ml) and TNFα (25 ng/ml) for indicated time with or without pretreatment of Emricasan (50 µg/ml) for 1 hr. (E, F) Immunoblot SP1 protein in eWAT (E) and iWAT (F) of WT and C6KO mice fed HFD for 12 weeks (n = 3). Two tailed unpaired Student's t ‐test. (G) In vitro cleavage of recombinant SP1 by active caspase‐6. Coomassie blue staining of gel. (H) Immunoblot of lysates from 3T3‐L1 adipocytes treated with cycloheximide (CHX, 5 µg/ml) and TNFα (25 ng/ml) for indicated time with or without pretreatment of Emricasan (50 µg/ml) for 1 hr. (I) Immunoblot of lysates from ex vivo cultured eWAT from WT or C6KO, treated with CHX (15 µg/ml) and TNFα (75 ng/ml) for indicated time. (J) Pnpla2 expression in 3T3‐L1 adipocytes treated with Rosiglitazone (5µM) or T0070907 (100 nM) in the presence or absence of Mithramycin (10 µM) for 6 h (n = 3). Two tailed unpaired Student's t ‐test. (K) In vitro cleavage of WT and mutant (D69E) PPARγ2 expressed in HEK293T by recombinant active caspase‐6. (L) In vitro cleavage of WT and mutant (D185E) SP1 expressed in HEK293T by recombinant active caspase‐6. (M) PPARγ activity reporter assay in HEK293T cell expressing PPRE‐H2B‐eGFP along with WT PPARγ or PPARγ D69E mutant in the absence or presence of CHX‐TNFα treatment (n = 8). Two tailed unpaired Student's t ‐test. (N‐V) Pearson correlation between CASP6 and PPARγ target genes in human adipose tissues ( GSE245948 ): Correlation matrix (N), AQP7 (O), CPT1B (P), FADS2 (Q), ME3 (R), PLIN4 (S), PCK2 (T), SLC27A1 (U), and SLC27A4 (V). (n = 76). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Recombinant active caspase‐6 was obtained from Enzo Life Sciences (#BML‐SE170), recombinant PPARγ from Sino Biological (#12019‐H20B), and recombinant SP1 from Active Motif (#81181).

Techniques: Control, Expressing, Western Blot, Two Tailed Test, In Vitro, Recombinant, Staining, Ex Vivo, Cell Culture, Mutagenesis, Activity Assay, Reporter Assay

Adipocyte‐specific caspase‐6 knockout protects against diet‐induced obesity and insulin resistance. Flox and adipocyte‐specific caspase‐6 knockout (ACKO) mice fed 60% HFD for 12 weeks. (A) Schematic diagram of generating Casp6 flox (Flox) mice and adipocyte‐specific Casp6 knockout (ACKO) mice. (B) Immunoblot of caspase‐6 protein in adipose tissues and livers. (C) Body weight (n = 9‐11). (D‐F) Tissue weights (n = 9‐11): eWAT (D), iWAT (E), BAT (F). Two tailed unpaired Student's t ‐test. (G–I) Indirect calorimetry (n = 6): oxygen consumption rate (G), carbon dioxide production (H), and energy expenditure (I). ANCOVA analysis with body weight as a covariate. (J, K) GTT (J, n = 9‐10) and ITT (K, n = 8‐9) with AUC quantification. GTT/ITT: two‐way ANOVA followed by Šídák's‐corrected post hoc test. (L) Immunoblots for the expression levels of PPARγ, SP1 and ATGL in eWAT and iWAT of Flox and ACKO mice fed HFD for 12 weeks. (M) Graphical summary. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Advanced Science

Article Title: Caspase‐6 Controls Lipid and Energy Metabolism in Diet‐Induced Obesity

doi: 10.1002/advs.202514784

Figure Lengend Snippet: Adipocyte‐specific caspase‐6 knockout protects against diet‐induced obesity and insulin resistance. Flox and adipocyte‐specific caspase‐6 knockout (ACKO) mice fed 60% HFD for 12 weeks. (A) Schematic diagram of generating Casp6 flox (Flox) mice and adipocyte‐specific Casp6 knockout (ACKO) mice. (B) Immunoblot of caspase‐6 protein in adipose tissues and livers. (C) Body weight (n = 9‐11). (D‐F) Tissue weights (n = 9‐11): eWAT (D), iWAT (E), BAT (F). Two tailed unpaired Student's t ‐test. (G–I) Indirect calorimetry (n = 6): oxygen consumption rate (G), carbon dioxide production (H), and energy expenditure (I). ANCOVA analysis with body weight as a covariate. (J, K) GTT (J, n = 9‐10) and ITT (K, n = 8‐9) with AUC quantification. GTT/ITT: two‐way ANOVA followed by Šídák's‐corrected post hoc test. (L) Immunoblots for the expression levels of PPARγ, SP1 and ATGL in eWAT and iWAT of Flox and ACKO mice fed HFD for 12 weeks. (M) Graphical summary. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Recombinant active caspase‐6 was obtained from Enzo Life Sciences (#BML‐SE170), recombinant PPARγ from Sino Biological (#12019‐H20B), and recombinant SP1 from Active Motif (#81181).

Techniques: Knock-Out, Western Blot, Two Tailed Test, Expressing

Structures of DBDF2,6T and hPPAR γ protein: (a) 2D structure of DBDF2,6T and (b) 3D structure of PPAR γ (4a4w.pdb).

Journal: Bioinorganic Chemistry and Applications

Article Title: Exploration on the Interaction Ability of Antitumor Compound Bis-[2,6-difluoro- N -(hydroxyl-<κ> O )benzamidato-<κ> O ]dibutylitin(IV) with Human Peroxisome Proliferator-Activated Receptor hPPAR γ

doi: 10.1155/2018/3063271

Figure Lengend Snippet: Structures of DBDF2,6T and hPPAR γ protein: (a) 2D structure of DBDF2,6T and (b) 3D structure of PPAR γ (4a4w.pdb).

Article Snippet: The protein human PPAR γ (hPPAR γ ) was purchased from Flarebio Company (Flarebio Biotech LLC, Wu Han, China) and stored at −20°C.

Techniques: